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recombinant mouse il7  (PeproTech)


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    Structured Review

    PeproTech recombinant mouse il7
    Recombinant Mouse Il7, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+il7/ccl21+chemokine/pm40249077-193-39-42
    Average 90 stars, based on 1 article reviews
    recombinant mouse il7 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement
    Article Snippet: FTY720 , MCE , HY-12005. .. Recombinant mouse IL7 , Peprotech , #210-07. .. Recombinant mouse IL15 , Peprotech , #210-15.

    Article Title: Signalling input from divergent pathways subverts B cell transformation.
    Article Snippet: Bone-marrow cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM, Gibco) with GlutaMAX supplemented with 20% FBS, 100 IU ml−1 penicillin, 100 μg ml−1 streptomycin and 50 μmol l−1 β-mercaptoethanol (Gibco). .. Bone-marrow cells, for IL7-dependent pro-B-cell culture, were cultured in full IMDM supplemented with 10 ng ml−1 recombinant mouse IL7 (PeproTech). ..

    Article Title: Combined Deletion of ZFP36L1 and ZFP36L2 Drives Superior Cytokine Production in T Cells at the Cost of Cell Fitness.
    Article Snippet: Erythrocytes were lysed using cold red blood cell lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA, pH 7.4) for 60 s. OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol. .. To generate effector OT-I T cells, purified OT-I T cells (1e6 cells/well) were activated with preseeded MEC.B7.SigOVA cells (1e5 cells/well) for 20 h. T cells were removed from stimulation and cultured in a complete medium supplemented with 10 ng/mL recombinant mouse IL7 (Peprotech) for 3–5 days as previously described [41]. ..

    other:

    Article Title: Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse NRP-1-PerCP5.5 Biolegend Cat# 145208, RRID:AB_2562034 Anti-mouse PD-L1-PerCP5.5 Biolegend Cat# 124334, RRID:AB_2629831 Anti-mouse CD11b-FITC Biolegend Cat# 101206, RRID:AB_312788 Anti-mouse CD11b-APC Biolegend Cat# 101212, RRID:AB_312795 Anti-mouse CD11c-FITC Biolegend Cat# 117306, RRID:AB_313775 Anti-mouse F4/80-FITC Biolegend Cat# 123108, RRID:AB_893502 Anti-mouse MHC II-APC Biolegend Cat# 107614, RRID:AB_313329 Anti-mouse CD83-PE Biolegend Cat# 121508, RRID:AB_572015 Anti-mouse CD103-PerCP Biolegend Cat# 121406, RRID:AB_1133989 Anti-mouse CD86-PerCP Biolegend Cat# 105028, RRID:AB_893420 Anti-mouse CD163-PE Biolegend Cat# 156704, RRID:AB_2860724 Anti-mouse Ly6G-PE Biolegend Cat# 127608, RRID:AB_1186104 Anti-mouse Ly6G-PC7 Biolegend Cat# 127618, RRID:AB_1877262 Anti-mouse NRP-1 Biolegend Cat# 145201, RRID:AB_2561840 Chemicals, peptides, and recombinant proteins Recombinant Mouse PD-1 (HEK293, His) MCE HY-P73724 Recombinant human PD-1 (HEK293, His) MCE HY-P7396 Blinatumomab MCE HY-P9963 FTY720 MCE HY-12005 Recombinant mouse IL7 Peprotech #210-07 Recombinant mouse IL15 Peprotech #210-15 Dynabeads for T cell activation/expansion TM Mouse T activator CD3/CD28 Gibco #11453D iRGD, c(CRGDKGPDC) Genescript Biotech Corporation N/A BCN-Cy5 Xi’an Ruixi Biological Technology Co., Ltd N/A N3-iRGD Nanjing Yuan Peptide Biotech Ltd. N/A OVA257-264 peptide Genscript Biotech Corporation N/A GP33 peptide Genscript Biotech Corporation N/A NY-ESO-1157-165 Genscript Biotech Corporation N/A collagenase IV Sigma-Aldrich C4-BIOC DNase I Sigma-Aldrich 04536282001 CFSE eBioscience #65-0850-84 Cell Proliferation Dye eFluorTM 670 eBioscience #65-0840-85 Cell Proliferation Dye eFluorTM 450 eBioscience #65-0842-90 CellTrackerTM Blue Invitrogen #C12881 Deep Red cell tracer Invitrogen #C37608 Zombie AquaTM Fixable Viability Kit Biolegend #423101 Critical commercial assays Cytofix/CytopermTM Fixation/Permeabilization Kit BD Biosciences #554714 Foxp3/transcription factor flow cytometry fixed membrane breaking buffer EBioscience #00-5523-00 GEXSCOPE Single Cell RNA-seq Kit Singleron Biotechnologies N/A Deposited data Mouse CD45+ cells single cell RNA-seq data This paper CNGB database: CNP0005642.

    Cell Culture:

    Article Title: Signalling input from divergent pathways subverts B cell transformation.
    Article Snippet: Bone-marrow cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM, Gibco) with GlutaMAX supplemented with 20% FBS, 100 IU ml−1 penicillin, 100 μg ml−1 streptomycin and 50 μmol l−1 β-mercaptoethanol (Gibco). .. Bone-marrow cells, for IL7-dependent pro-B-cell culture, were cultured in full IMDM supplemented with 10 ng ml−1 recombinant mouse IL7 (PeproTech). ..

    Article Title: Combined Deletion of ZFP36L1 and ZFP36L2 Drives Superior Cytokine Production in T Cells at the Cost of Cell Fitness.
    Article Snippet: Erythrocytes were lysed using cold red blood cell lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA, pH 7.4) for 60 s. OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol. .. To generate effector OT-I T cells, purified OT-I T cells (1e6 cells/well) were activated with preseeded MEC.B7.SigOVA cells (1e5 cells/well) for 20 h. T cells were removed from stimulation and cultured in a complete medium supplemented with 10 ng/mL recombinant mouse IL7 (Peprotech) for 3–5 days as previously described [41]. ..

    Purification:

    Article Title: Combined Deletion of ZFP36L1 and ZFP36L2 Drives Superior Cytokine Production in T Cells at the Cost of Cell Fitness.
    Article Snippet: Erythrocytes were lysed using cold red blood cell lysis buffer (155 mM NH4Cl, 10 mM KHCO3, 0.1 mM EDTA, pH 7.4) for 60 s. OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol. .. To generate effector OT-I T cells, purified OT-I T cells (1e6 cells/well) were activated with preseeded MEC.B7.SigOVA cells (1e5 cells/well) for 20 h. T cells were removed from stimulation and cultured in a complete medium supplemented with 10 ng/mL recombinant mouse IL7 (Peprotech) for 3–5 days as previously described [41]. ..



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    FIGURE 2. Nemo inactivation leads to increased RAG cleavage of Igk loci. (A) Schematic for Southern analysis of Igk cleavage by RAG indicating the GL configuration of the Jj region of the Igk locus with flanking EcoRI and SacI restriction sites, the GL Jj region generated by EcoR1 and SacI digestion, and each Jj CE generated by RAG cleavage. (B) Representative Southern blot analysis of Jj cleavage and (C) quantification of the remaining GL Jj fragment in primary preB cell cultures from BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA) and Mb1Cre1:BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA:Nemo−/−) mice. Cells were collected after 4 d of culture in <t>IL-7</t> or at 24, 48, or 72 h after IL-7 withdrawal of cultures treated with or without the ATM inhibitor (ATMi) KU55933 (15 lM). The probe 39 of the Jj region depicts the GL Jj fragment and indicated Jj CEs. Southern blot using a probe for Hprt was used to normalize DNA content in each lane. The quantification is the amount of GL Jj relative to Hprt and normalized to the BIA IL-71 sample. These data are from one indepen- dent experiment. (D) Schematic of TaqMan quantification of GL Jj indicating the relative positions of the primers and TaqMan probe used for each Jj gene segment. (E) TaqMan quantification of GL Jj1 from the same samples as the ones used for the Southern blot analysis in (B) and (C). TaqMan quantification of the Cd19 locus was used to normalize DNA content. The Jj1 value was first normalized to Cd19 and then to the BIA IL-71 sample. These data are from one independent experiment. (F) TaqMan quantification of GL Jj1 from an aliquot of the same samples as Fig. 1. These samples are from primary preB cell cultures from BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1), Mb1Cre1:BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1:Nemo−/−), BCL2:IgH:Artemis−/−:Nemoflox/flox
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    FIGURE 2. Nemo inactivation leads to increased RAG cleavage of Igk loci. (A) Schematic for Southern analysis of Igk cleavage by RAG indicating the GL configuration of the Jj region of the Igk locus with flanking EcoRI and SacI restriction sites, the GL Jj region generated by EcoR1 and SacI digestion, and each Jj CE generated by RAG cleavage. (B) Representative Southern blot analysis of Jj cleavage and (C) quantification of the remaining GL Jj fragment in primary preB cell cultures from BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA) and Mb1Cre1:BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA:Nemo−/−) mice. Cells were collected after 4 d of culture in <t>IL-7</t> or at 24, 48, or 72 h after IL-7 withdrawal of cultures treated with or without the ATM inhibitor (ATMi) KU55933 (15 lM). The probe 39 of the Jj region depicts the GL Jj fragment and indicated Jj CEs. Southern blot using a probe for Hprt was used to normalize DNA content in each lane. The quantification is the amount of GL Jj relative to Hprt and normalized to the BIA IL-71 sample. These data are from one indepen- dent experiment. (D) Schematic of TaqMan quantification of GL Jj indicating the relative positions of the primers and TaqMan probe used for each Jj gene segment. (E) TaqMan quantification of GL Jj1 from the same samples as the ones used for the Southern blot analysis in (B) and (C). TaqMan quantification of the Cd19 locus was used to normalize DNA content. The Jj1 value was first normalized to Cd19 and then to the BIA IL-71 sample. These data are from one independent experiment. (F) TaqMan quantification of GL Jj1 from an aliquot of the same samples as Fig. 1. These samples are from primary preB cell cultures from BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1), Mb1Cre1:BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1:Nemo−/−), BCL2:IgH:Artemis−/−:Nemoflox/flox
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    STEMCELL Technologies Inc cytokines (mouse recombinant scf, gm-csf, il3, il6, il7)
    FIGURE 2. Nemo inactivation leads to increased RAG cleavage of Igk loci. (A) Schematic for Southern analysis of Igk cleavage by RAG indicating the GL configuration of the Jj region of the Igk locus with flanking EcoRI and SacI restriction sites, the GL Jj region generated by EcoR1 and SacI digestion, and each Jj CE generated by RAG cleavage. (B) Representative Southern blot analysis of Jj cleavage and (C) quantification of the remaining GL Jj fragment in primary preB cell cultures from BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA) and Mb1Cre1:BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA:Nemo−/−) mice. Cells were collected after 4 d of culture in <t>IL-7</t> or at 24, 48, or 72 h after IL-7 withdrawal of cultures treated with or without the ATM inhibitor (ATMi) KU55933 (15 lM). The probe 39 of the Jj region depicts the GL Jj fragment and indicated Jj CEs. Southern blot using a probe for Hprt was used to normalize DNA content in each lane. The quantification is the amount of GL Jj relative to Hprt and normalized to the BIA IL-71 sample. These data are from one indepen- dent experiment. (D) Schematic of TaqMan quantification of GL Jj indicating the relative positions of the primers and TaqMan probe used for each Jj gene segment. (E) TaqMan quantification of GL Jj1 from the same samples as the ones used for the Southern blot analysis in (B) and (C). TaqMan quantification of the Cd19 locus was used to normalize DNA content. The Jj1 value was first normalized to Cd19 and then to the BIA IL-71 sample. These data are from one independent experiment. (F) TaqMan quantification of GL Jj1 from an aliquot of the same samples as Fig. 1. These samples are from primary preB cell cultures from BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1), Mb1Cre1:BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1:Nemo−/−), BCL2:IgH:Artemis−/−:Nemoflox/flox
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    FIGURE 2. Nemo inactivation leads to increased RAG cleavage of Igk loci. (A) Schematic for Southern analysis of Igk cleavage by RAG indicating the GL configuration of the Jj region of the Igk locus with flanking EcoRI and SacI restriction sites, the GL Jj region generated by EcoR1 and SacI digestion, and each Jj CE generated by RAG cleavage. (B) Representative Southern blot analysis of Jj cleavage and (C) quantification of the remaining GL Jj fragment in primary preB cell cultures from BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA) and Mb1Cre1:BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA:Nemo−/−) mice. Cells were collected after 4 d of culture in IL-7 or at 24, 48, or 72 h after IL-7 withdrawal of cultures treated with or without the ATM inhibitor (ATMi) KU55933 (15 lM). The probe 39 of the Jj region depicts the GL Jj fragment and indicated Jj CEs. Southern blot using a probe for Hprt was used to normalize DNA content in each lane. The quantification is the amount of GL Jj relative to Hprt and normalized to the BIA IL-71 sample. These data are from one indepen- dent experiment. (D) Schematic of TaqMan quantification of GL Jj indicating the relative positions of the primers and TaqMan probe used for each Jj gene segment. (E) TaqMan quantification of GL Jj1 from the same samples as the ones used for the Southern blot analysis in (B) and (C). TaqMan quantification of the Cd19 locus was used to normalize DNA content. The Jj1 value was first normalized to Cd19 and then to the BIA IL-71 sample. These data are from one independent experiment. (F) TaqMan quantification of GL Jj1 from an aliquot of the same samples as Fig. 1. These samples are from primary preB cell cultures from BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1), Mb1Cre1:BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1:Nemo−/−), BCL2:IgH:Artemis−/−:Nemoflox/flox

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Nemo-Dependent, ATM-Mediated Signals from RAG DNA Breaks at Igk Feedback Inhibit V κ Recombination to Enforce Igκ Allelic Exclusion.

    doi: 10.4049/jimmunol.2100696

    Figure Lengend Snippet: FIGURE 2. Nemo inactivation leads to increased RAG cleavage of Igk loci. (A) Schematic for Southern analysis of Igk cleavage by RAG indicating the GL configuration of the Jj region of the Igk locus with flanking EcoRI and SacI restriction sites, the GL Jj region generated by EcoR1 and SacI digestion, and each Jj CE generated by RAG cleavage. (B) Representative Southern blot analysis of Jj cleavage and (C) quantification of the remaining GL Jj fragment in primary preB cell cultures from BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA) and Mb1Cre1:BCL2:IgH:Artemis−/−:Nemoflox/flox (BIA:Nemo−/−) mice. Cells were collected after 4 d of culture in IL-7 or at 24, 48, or 72 h after IL-7 withdrawal of cultures treated with or without the ATM inhibitor (ATMi) KU55933 (15 lM). The probe 39 of the Jj region depicts the GL Jj fragment and indicated Jj CEs. Southern blot using a probe for Hprt was used to normalize DNA content in each lane. The quantification is the amount of GL Jj relative to Hprt and normalized to the BIA IL-71 sample. These data are from one indepen- dent experiment. (D) Schematic of TaqMan quantification of GL Jj indicating the relative positions of the primers and TaqMan probe used for each Jj gene segment. (E) TaqMan quantification of GL Jj1 from the same samples as the ones used for the Southern blot analysis in (B) and (C). TaqMan quantification of the Cd19 locus was used to normalize DNA content. The Jj1 value was first normalized to Cd19 and then to the BIA IL-71 sample. These data are from one independent experiment. (F) TaqMan quantification of GL Jj1 from an aliquot of the same samples as Fig. 1. These samples are from primary preB cell cultures from BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1), Mb1Cre1:BCL2:IgH:Rag1−/−:Nemoflox/flox (BIR1:Nemo−/−), BCL2:IgH:Artemis−/−:Nemoflox/flox

    Article Snippet: These bone marrow cells were cultured for 4 d in RPMI 1640 supplemented with 10% FBS, 10 mM HEPES, 13 nonessential amino acids, 1 mM L-glutamine, 1 mM sodium pyruvate, 100 U/ml penicillin streptomycin, 50 mM 2-ME, and 5 ng/ml IL7 (407-ML; R&D Systems).

    Techniques: Generated, Southern Blot